BTX Microslides are used primarily with the ECM® 2001 Electro CellManipulator. The Microslide fits on the microscope allowingobservation of the alignment and fusion process. The Microslide 450is composed of a glass microslide and two strips of 0.5 mmstainless steel tubing which functions as electrodes to provide adivergent field. These wires are set apart at different gaps toprovide various field strengths and volumes. The Micrograbber isused to connect the Microslide 450 to the Coaxial Connection Cable,which will then be hooked up to the ECM 2001 generator. Microslide453 has two stainless steel square bars that are set apart atvarious gaps to provide homogeneous fields. The Square Post Cableis used to connect the Microslide to the Coaxial Connection Cable,which will be hooked up to the ECM 2001 generator.
The Microslides have been used for embryo manipulation1 and cell orprotoplast fusion2. Advantage of the Microslide is that the processof dimer formation can be observed under the microscope.
Standard Capabilities *Depending on buffer composition andgenerator capability
Voltage Range: 0 - 500 Vdc
Pulse Length/Time Constants Range: 1 μsec - 99 msec
Pulse Number Range: 1-99 (depending on voltage)
Operating Temperature: 5° - 40® C
Intended Use: Indoor Use
Relative Humidity: 20-80%
Maximum Altitude: 2000m (6562 ft)
Pollution Degree: II
Insulation Category: CAT I
Physical Characteristics 450 450-1 453 453-10
Gap Size: 0.5 m 1.0 mm 3.2 mm 10 mm
Volume: 20 μl 40 μl 0.7 ml 2.2 ml
Autoclavable: No No No No
Generators ECM 630, 830, 395, 399, 2001, 600, & T820.
Model Description Part Number
450 Microslide 20 μl - package of 10, 0.5mm gap 01-000209-01
450-1 Microslide 40 μl - package of 10, 1.0mm gap 01-000210-01
453 Microslide 3.2 mm gap - package of 1 01-000211-01
453-10 Microslide 10 mm gap - package of 1 01-000212-01
5343 Coaxial Banana Plug Connection Cable, 10 ft 06-700042-01
465 Square Post Cables 06-700044-01
BTX Protocol PR0117, Personal communication with Dr. James Robl,University of Massachusetts.
Saunders, J.A. and Bates, G.W., Guide to Electroporation andElectrofusion, Academic Press, p. 477-479, 1991